التأثيرات الناتجة عن استخدام عقاري الديكلوفيناك والسيبروفلوكساسين على الكبد والكلى لدى الجرذان
محتوى المقال
الملخص
جرى في هذه الدراسة تقييم التأثيرات السُمية لديكلوفيناك الصوديوم (DCF) والسيبروفلوكساسين (CIP) على التعبير الجيني للسيتوكروم بي 450 أكسيداز ( (CYPs وعلى أنسجة الكبد والكلى لدى ذكور الجرذان البيضاء. واختير عقارا ديكلوفيناك الصوديوم (DCF) والسيبروفلوكساسين (CIP) نظرًا لأنهما مثبطان لأنزيمات معينة من سيتوكروم بي 450 أكسيداز (CYPs-P450). وتم تقسيم حيوانات التجربة (35 من ذكور الجرذان البيضاء البالغة) إلى 7 مجموعات (A, B, C, D, E, F, G) تتألف كل منها من 5 أفراد، وتم إعطاؤها العقارين عن طريق الفم لمدة 21 يومًا متتاليًا. المجموعة (A) هي المجموعة الضابطة، بينما تم إعطاء الجرذان في المجموعتين (B) و (C) جرعة ديكلوفيناك الصوديوم (DCF) بمقدار 5.3 و 10.6 ملغم/كغم من وزن الجسم على التوالي. في حين تم إعطاء أفراد المجموعتين (D) و (E) جرعة سيبروفلوكساسين (CIP) بمقدار 40 و 80 ملغم/كغم من وزن الجسم على التوالي. وتم إعطاء أفراد المجموعتين (F) و (G) مزيجًا من الجرعات القليلة والجرعات الكبيرة من كلا العقارين على التوالي. خفَّضَ كل من العقارين إلى حد كبير من تعبير الحمض النووي الريبي المرسال (mRNA) لكل من السيتوكروم CYP1A2 والسيتوكروم CYP3A4 والسيتوكروم CYP2C9. كما تسبب العقاران بحدوث تغييرات نسيجية في الكبد والكلى. في الكبد تمت ملاحظة وجود تليف كبير، ونخر، وتسلل الخلايا الالتهابية، ونزيف، وتنكس مائي. وفي الكلى تمت ملاحظة وجود إصابة بالغة في الأنسجة، بالإضافة إلى تلف أنبوبي وكبيبي بسبب النخر الشديد، وتنكس الخلايا الالتهابية المرافقة، وكذلك وجود احتقان للأوعية الدموية. وعلى الرغم من استمرار استخدام ديكلوفيناك الصوديوم (DCF) والسيبروفلوكساسين (CIP) كعقاقير علاجية، فإن استخدامهما يجب أن يكون محدودًا؛ لأن إعطاؤهما بشكل مزمن له تأثير سُمي على صحة البشر.
الكلمات المفتاحية
علوم الأدلة الجنائية، السُمية، السيتوكروم بي 450 أكسيداز، علم أمراض الأنسجة، العقاقير المضادة للالتهابات
التنزيلات
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