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Lakshmi Sasikumar Panicker Fayth D'Silva Shivani Satish Surve

Abstract

In sexual assaults the presence of semen on the crime scene, victim or suspect’s belongings acts as crucial evidence for establishing the occurrence of the crime. Fabrics are a common piece of evidence obtained at such crime scenes. The detection of seminal stain on a fabric depends on various factors such as absorption by the fabric, the colour and texture of fabric, the age of the stain, environmental conditions, etc. The current study was conducted on three different types of fabrics: cotton, nylon and denim and were exposed to a temperature of 25˚C for four different time durations- 1 day (24 hours), 3 days (72 hours), 5 days (120 hours) and 7 days (168 hours). Three different tests were performed on the samples to be tested. Accordingly, fluorescence test, Barberio’s test and Christmas tree staining test were carried out. It was determined that the fluorescence of the seminal stain increased with increasing time duration. The observations made in Barberio test was however, not seen to be affected by the time intervals. It was also observed that the sperm density was affected with increase of time of incubation of the seminal stains.

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References

Inman K, Rudin N, Barnett PD, Lentini JJ. Human Biological Evidences. In: Scientific Protocols for Forensic Examination of Clothing. 2011th ed. CRC Press; 2011. p. 123–44.

Schlagetter TG, Glynn CL. The Effect of Fabric Type and Laundering Conditions on the Detection of Semen Stains. Int J Forensic Sci [Internet]. 2017;2(2). Available from: http://digitalcommons.newhaven.edu/forensicscience-facpubs

Kobus, H., Silenieks, E. and Scharnberg J. Improving the effectiveness of fluorescence for the detection of semen stains on fabric. J Forensic Sci. 2002;47(4):1–5.

Zou Y, Xia P, Yang F, Cao F, Ma K, Mi Z, et al. Whole blood and semen identification using mid-infrared and Raman spectrum analysis for forensic applications. Anal Methods. 2016;8(18):3763–7.

Achetib N, Wilk LS, Schwarz JCV, Lambrechts SAG, Van Leeuwen TG, Aalders MCG, et al. Estimating the Time of Deposition of Semen Traces using Fluorescence Protein-Lipid Oxidation Signatures. Anal Chem. 2019;91(5):3204–8.

Zha S, Wei X, Fang R, Wang Q, Lin H, Zhang K, et al. Estimation of the age of human semen stains by attenuated total reflection Fourier transform infrared spectroscopy: a preliminary study. Forensic Sci Res [Internet]. 2020;5(2):119–25. Available from: https://doi.org/10.1080/20961790.2019.1642567

McLaughlin G, Fikiet MA, Ando M, Hamaguchi H o., Lednev IK. Universal detection of body fluid traces in situ with Raman hyperspectroscopy for forensic purposes: Evaluation of a new detection algorithm (HAMAND) using semen samples. J Raman Spectrosc [Internet]. 2019;50(8):1147–53. Available from: https://doi.org/10.1002/jrs.5621

Das T, Harshey A, Srivastava A, Nigam K, Yadav VK, Sharma K, et al. Analysis of the ex-vivo transformation of semen, saliva and urine as they dry out using ATR-FTIR spectroscopy and chemometric approach. Sci Rep [Internet]. 2021;11(1):1–10. Available from: https://doi.org/10.1038/s41598-021-91009-5

Ballantyne, Jack. Determination of the age (time since deposition) of a biological stain. US Department of Justice, 2009.

Hackshaw K V., Miller JS, Aykas DP, Rodriguez-Saona L. Vibrational spectroscopy for identification of metabolites in biologic samples. Molecules. 2020;25(20).

Miranda GE, Prado FB, Delwing F, Daruge E. Analysis of the fluorescence of body fluids on different surfaces and times. Sci Justice. 2014;54(6):427–31.

Young J. Seminal stain fluorescence using three alternate light source-barrier filter combinations on six different colors of cotton fabrics [Internet]. 2015. Available from: http://hdl.handle.net/2144/16197

True RH. THE HARMFUL ACTION OF DISTILLED WATER. Am J Bot [Internet]. 1914 Jun 1;1(6):255–73. Available from: https://doi.org/10.1002/j.1537-2197.1914.tb05392.x

Littlejohn H, Pirie J. The Micro-Chemical Tests for Semen. Edinb Med J. 23:410–7.

Davidson G, Jalowiecki TB. Acid phosphatase screening - Wetting test paper or wetting fabric and test paper? Sci Justice. 2012 Jun;52(2):106–11.

Gonçalves ABR, de Oliveira CF, Carvalho EF, Silva DA. Comparison of the sensitivity and specificity of colorimetric and immunochromatographic presumptive methods for forensic semen detection. Forensic Sci Int Genet Suppl Ser. 2017 Dec 1;6:e481–3.

Lewis J, Baird A, McAlister C, Siemieniuk A, Blackmore L, McCabe B, et al. Improved detection of semen by use of direct acid phosphatase testing. Sci Justice. 2013 Dec;53(4):385–94.

Kolowski J. Microscopic Examination of Spermatozoa by Christmas Tree stain [Internet]. 2014. p. 1–6. Available from: https://dfs.dc.gov/sites/default/files/dc/sites/dfs/page_content/attachments/FBS07 Sperm Search.pdf

Soules MR, Pollard AA, Brown KM, Verma M. The forensic laboratory evaluation of evidence in alleged rape. Am J Obstet Gynecol. 1978 Jan 15;130(2):142–7.

Suttipasit P, Wongwittayapanich S. Detection of prostate specific antigen and semenogelin in specimens from female rape victims. J Forensic Leg Med. 2018 Feb 1;54:102–8.

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