Qualitative Analysis of Post-blast Residue using the Double Hyphenated UHPLC-(HESI)-MS/MS Technique
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Abstract
In this paper, the identification and forensic analysis of post-blast residues recovered from controlled blast sites has been presented. The targeted sample was extracted from post-blast soil by using the Accelerated Solvent Extraction technique. The average recovery varies from 86-93% at 250, 500,750 and 1000 ng/g concentration level. The target compound was primarily identified to be PETN (Penta Erythritol Tetra Nitrate) by a color test and TLC (Thin Layer Chromatography). The confirmatory test for the target analyte was done by using LC-MS/MS (Liquid Chromatography - Tandem Mass Spectrometry). Heated Electron Spray Ionization (HESI) interface with negative mode was employed and SRM (Selected Reaction Monitoring) on Triple Quadruple Mass Analyzer was used for confirmation of PETN in the sample. The Limit of Detection (LOD) obtained from the analysis of post-blast residue by using this method was 7.9 ng/g. This manuscript demonstrates the viability of LC-MS/MS for a fast, accurate and quality-assured analysis of post-blast residue.
Keywords
Accelerated Solvent Extraction, Explosives, Forensic Sciences, Post-blast residue, LC-MS/MS
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Lambert E, Nerbonne T, Watson PL, Buss J, Clarke A, Hogan N, Barton S, Lambert J. The forensic science needs of law enforcement applicants and recruits: A survey of Michigan law enforcement agencies. Journal of Criminal Justice Education. 2003 Mar 1;14(1):67-81. https://doi.org/10.1080/10511250300085661
Kaplan J, Ling S, Cuellar M. Public beliefs about the accuracy and importance of forensic evidence in the United States. Science & Justice. 2020 May 1;60(3):263-72. https://doi.org/10.1016/j.scijus.2020.01.001
Johns C, Shellie RA, Potter OG, O’Reilly JW, Hutchinson JP, Guijt RM, Breadmore MC, Hilder EF, Dicinoski GW, Haddad PR. Identification of homemade inorganic explosives by ion chromatographic analysis of post-blast residues. Journal of Chromatography A. 2008 Feb 29;1182(2):205-14. https://doi.org/10.1016/j.chroma.2008.01.014
Benson SJ, Lennard CJ, Maynard P, Hill DM, Andrew AS, Roux C. Forensic analysis of explosives using isotope ratio mass spectrometry (IRMS)—Preliminary study on TATP and PETN. Science & Justice. 2009 Jun 1;49(2):81-6. https://doi.org/10.1016/j.scijus.2009.03.004
Benson SJ, Lennard CJ, Maynard P, Hill DM, Andrew AS, Roux C. Forensic analysis of explosives using isotope ratio mass spectrometry (IRMS)—Discrimination of ammonium nitrate sources. Science & Justice. 2009 Jun 1;49(2):73-80. https://doi.org/10.1016/j.scijus.2009.04.005
Marshall M. Post-blast detection issues. InAspects of Explosives Detection 2009 Jan 1 (pp. 223-243). Elsevier. https://doi.org/10.1016/B978-0-12-374533-0.00011-8
Banas A, Banas K, Bahou M, Moser HO, Wen L, Yang P, Li ZJ, Cholewa M, Lim SK, Lim CH. Post-blast detection of traces of explosives by means of Fourier transform infrared spectroscopy. Vibrational Spectroscopy. 2009 Nov 10;51(2):168-76. https://doi.org/10.1016/j.vibspec.2009.04.003
Huri MA, Ahmad UK, Ibrahim R, Omar M. A review of explosive residue detection from forensic chemistry perspective. Malaysian J. Anal. Sci. 2017;21(2):267-82. https://doi.org/10.17576/mjas-2017-2102-01
Black C, D'Souza T, Smith JC, Hearns NG. Identification of post-blast explosive residues using direct-analysis-in-real-time and mass spectrometry (DART-MS). Forensic Chemistry. 2019 Dec 1;16:100185. https://doi.org/10.1016/j.forc.2019.100185
Martín-Alberca C, Zapata F, Carrascosa H, Ortega-Ojeda FE, García-Ruiz C. Study of consumer fireworks post-blast residues by ATR-FTIR. Talanta. 2016 Mar 1;149:257-65. https://doi.org/10.1016/j.talanta.2015.11.070
Schachel TD, Stork A, Schulte-Ladbeck R, Vielhaber T, Karst U. Identification and differentiation of commercial and military explosives via high performance liquid chromatography–high resolution mass spectrometry (HPLC-HRMS), X-ray diffractometry (XRD) and X-ray fluorescence spectroscopy (XRF): Towards a forensic substance database on explosives. Forensic science international. 2020 Mar 1;308:110180. https://doi.org/10.1016/j.forsciint.2020.110180
Sanchez C, Carlsson H, Colmsjö A, Crescenzi C, Batlle R. Determination of nitroaromatic compounds in air samples at femtogram level using C18 membrane sampling and on-line extraction with LC− MS. Analytical chemistry. 2003 Sep 1;75(17):4639-45. https://doi.org/10.1021/ac034278w
Sisco E, Najarro M, Samarov D, Lawrence J. Quantifying the stability of trace explosives under different environmental conditions using electrospray ionization mass spectrometry. Talanta. 2017 Apr 1;165:10-7. https://doi.org/10.1016/j.talanta.2016.12.029
Kober SL, Hollert H, Frohme M. Quantification of nitroaromatic explosives in contaminated soil using MALDI-TOF mass spectrometry. Analytical and bioanalytical chemistry. 2019 Sep;411(23):5993-6003. https://doi.org/10.1007/s00216-019-01976-y
Mäkinen M, Nousiainen M, Sillanpää M. Ion spectrometric detection technologies for ultra‐traces of explosives: A review. Mass spectrometry reviews. 2011 Sep;30(5):940-73. https://doi.org/10.1002/mas.20308
Almog J, Zitrin S. Colorimetric detection of explosives. InAspects of Explosives Detection 2009 Jan 1 (pp. 41-58). Elsevier. https://doi.org/10.1016/B978-0-12-374533-0.00004-0
Yinon J. Explosives. InHandbook of Analytical Separations 2000 Jan 1 (Vol. 2, pp. 603-616). Elsevier Science BV. https://doi.org/10.1016/S1567-7192(00)80072-8
Ong TH, Mendum T, Geurtsen G, Kelley J, Ostrinskaya A, Kunz R. Use of mass spectrometric vapor analysis to improve canine explosive detection efficiency. Analytical Chemistry. 2017 Jun 20;89(12):6482-90. https://doi.org/10.1021/acs.analchem.7b00451
Yinon J. Analysis and detection of explosives by mass spectrometry. InAspects of explosives detection 2009 Jan 1 (pp. 147-169). Elsevier. https://doi.org/10.1016/B978-0-12-374533-0.00008-8
Verkouteren JR, Staymates JL. Reliability of ion mobility spectrometry for qualitative analysis of complex, multicomponent illicit drug samples. Forensic science international. 2011 Mar 20;206(1-3):190-6. https://doi.org/10.1016/j.forsciint.2010.08.005.
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