Quantifying the Relationship Between Skin Temperature and Skin Hydration Measured by Corneometry
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Abstract
The role of the skin in forensic medicine is increasingly recognised, particularly in post mortem interval (PMI) determination through its electrical properties. This study investigates the relationship between stratum corneum hydration (SCH), measured by corneometry, and skin temperature (ST), as literature strongly suggests that variations in skin temperature can influence its electrical properties. Twenty-four (24) adult subjects with known cause of death and characteristics were selected and briefly removed from their mortuary cell. ST (°C) and SCH (a.u.) were measured immediately and then at 5-minute intervals for 20 minutes using the Skin-Thermometer ST 500® and Corneometer CM-825® (Courage & Khazaka electronic GmbH, Cologne, Germany) at 3 body sites (cheekbone, abdomen and index finger). The results show a significant inverse relationship between ST and SCH, with a unit decrease in SCH for each unit increase in ST (-1.21 ± 0.55, p=0.028). This relationship was site-dependent (p <0.0001) but not influenced by time since removal from the mortuary cell (RMCT) (p = 0.15), sex (p = 0.39) or PMI (p = 0.88). The results highlight the need for careful consideration of skin temperature when assessing skin hydration, in order to accurately interpret post mortem changes in skin electrical properties, particularly when determining the post mortem interval (PMI).
Keywords
Forensic sciences, post mortem interval, skin temperature, skin hydration, skin capacitance, corneometer CM-825®
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De-Giorgio F, Bergamin E, Baldi A, Gatta R, Pascali VL. Immunohistochemical expression of HMGB1 and related proteins in the skin as a possible tool for determining post-mortem interval: a preclinical study. Forensic Sci Med Pathol. 2023 Jul 25;20(1):149–65.
Xie DG, Wang XM, Li JH, Tan ZY, Zhang ZQ, Li ST. Short-term postmortem interval estimation by detection of apoptosis-related protein in skin. Forensic Sci Med Pathol. 2024 Jan 30;20(3):872–7.
Ali MM, Ibrahim SF, Fayed AA. Using Skin Gene Markers for Estimating Early Postmortem Interval at Different Temperatures. Am J Forensic Med Pathol. 2017 Dec;38(4):323–5.
Ali MM, Ibrahim SF, Elrewieny NM, Elyamany AM, Khalil WKB, Shalby AB, et al. Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental Study. Biomedicines. 2022 Nov 14;10(11):2919.
Gonnade U, Chavan KD. Study of early postmortem changes in skin for estimation of postmortem interval at pims, loni. J Indian Acad Forensic Med. 2018;40(4):396–400.
Leccia C, Alunni V, Quatrehomme G. Modern (forensic) mummies: A study of twenty cases. Forensic Sci Int. 2018 Jul;288:330.e1-330.e9.
Connor M, Baigent C, Hansen ES. Measuring Desiccation Using Qualitative Changes: A Step Toward Determining Regional Decomposition Sequences. J Forensic Sci. 2019 Jul;64(4):1004–11.
Wei W, Michu Q, Wenjuan D, Jianrong W, Zhibing H, Ming Y, et al. Histological changes in human skin 32 days after death and the potential forensic significance. Sci Rep. 2020 Oct 30;10(1):18753.
Kovarik C, Stewart D, Cockerell C. Gross and Histologic Postmortem Changes of the Skin: Am J Forensic Med Pathol. 2005 Dec;26(4):305–8.
Bardale RV, Tumram NK, Dixit PG, Deshmukh AY. Evaluation of Histologic Changes of the Skin in Postmortem Period: Am J Forensic Med Pathol. 2012 Dec;33(4):357–61.
Garcidueñas ALC, Santiesteban GM, Rodríguez ED, Flores RMC, Rodríguez PB. Forensic study of skin postmortem changes as a supplementary test to determine postmortem interval (first 78 hours). 2016;3(2):27–33.
Gidado IM, Qassem M, Triantis IF, Kyriacou PA. Review of Advances in the Measurement of Skin Hydration Based on Sensing of Optical and Electrical Tissue Properties. Sensors. 2022 Sep 21;22(19):7151.
Morin M, Ruzgas T, Svedenhag P, Anderson CD, Ollmar S, Engblom J, et al. Skin hydration dynamics investigated by electrical impedance techniques in vivo and in vitro. Sci Rep. 2020 Oct 14;10(1):17218.
Ceciliason AS, Andersson MG, Lindström A, Sandler H. Quantifying human decomposition in an indoor setting and implications for postmortem interval estimation. Forensic Sci Int. 2018 Feb;283:180–9.
Ceciliason AS, Käll B, Sandler H. Mummification in a forensic context: an observational study of taphonomic changes and the post-mortem interval in an indoor setting. Int J Legal Med. 2023 Jul;137(4):1077–88.
Galloway A, Birkby W, Jones A, Henry T, Parks B. Decay rates of human remains in an arid environment. J Forensic Sci. 1989;34(3):607–16.
Partoune A, Donneau AF, Dardenne N, Boxho P. Estimation of Intra and Inter-Individual Variability of Post Mortem Skin Hydration by Corneometry. Arab J Forensic Sci Amp Forensic Med. 2024 Dec;6(2):115–40.
Barel AO, Clarys P. Skin Capacitance. In: Non Invasive Diagnostic Techniques in Clinical Dermatology [Internet]. Berlin: Springer; 2014 [cited 2022 Nov 14]. p. 357–66. Available from: http://link.springer.com/10.1007/978-3-642-32109-2
Clarys P, Clijsen R, Taeymans J, Barel AO. Hydration measurements of the stratum corneum: comparison between the capacitance method (digital version of the Corneometer CM 825®) and the impedance method (Skicon-200EX®). Skin Res Technol. 2012 Aug;18(3):316–23.
Heinrich U, Koop U, Leneveu-Duchemin MC, Osterrieder K, Bielfeldt S, Chkarnat C, et al. Multicentre comparison of skin hydration in terms of physical-, physiological- and product-dependent parameters by the capacitive method (Corneometer CM 825). Int J Cosmet Sci. 2003 Apr;25(1–2):45–53.
Courage+Khazaka electronic GmbH. Corneometer® CM 825 - Technical Data [Internet]. 2020 [cited 2020 Oct 11]. Available from: https://www.courage-khazaka.de/en/downloads-en?task=callelement&format=raw&item_id=318&element=f85c494b-2b32-4109-b8c1-083cca2b7db6&method=download&args[0]=87b62cd6bf1f9279c7088266a2569685
Querido D. Temperature-correction of abdominal impedance: improved relationship between impedance and postmortem interval. Forensic Sci Int. 2000 Mar;109(1):39–50.
Hansen ES, Baigent C, Reck SI, Connor M. Bioelectrical Impedance as a Technique for Estimating Postmortem Interval,,. J Forensic Sci. 2018 Jul;63(4):1186–90.
Berardesca E, European Group for Efficacy Measurements on Cosmetics and Other Topical Products (EEMCO)2. EEMCO guidance for the assessment of stratum corneum hydration: electrical methods. Skin Res Technol. 1997 May;3(2):126–32.
Clarys P, Clijsen R, Barel AO. Influence of probe application pressure on in vitro and in vivo capacitance (Corneometer CM 825®) and conductance (Skicon 200 EX®) measurements: Probe pressure during hydration measurements. Skin Res Technol. 2011 Nov;17(4):445–50.
W. Weber, K. Munzert. Postmortale Exsikkation der Fingerbeeren ? Ergebnisse systematischer quantitativer und qualitativer experimenteller Untersuchungen@@@Desiccation of fingertips post mortem ? Stage classification at experimental time intervals. 1986;96(4). Available from: http://dx.doi.org/10.1007/BF00200707
Lodén M, Hagforsen E, Lindberg M. The presence of body hair influences the measurement of skin hydration with the Corneometer. Acta Derm Venereol. 1995 Nov;75(6):449–50.
Barel AO, Clarys P. In vitro calibration of the capacitance method (Corneometer CM 825) and conductance method (Skicon-200) for the evaluation of the hydration state of the skin. Skin Res Technol. 1997 May;3(2):107–13.
Tagami H, Ohi M, Iwatsuki K, Kanamaru Y, Yamada M, Ichijo B. Evaluation of the Skin Surface Hydration in Vivo by Electrical Measurement. J Invest Dermatol. 1980 Dec;75(6):500–7.
Samadi A, Yazdanparast T, Shamsipour M, Hassanzadeh H, Hashemi Orimi M, Firooz R, et al. Stratum corneum hydration in healthy adult humans according to the skin area, age and sex: a systematic review and meta‐analysis. J Eur Acad Dermatol Venereol. 2022 Oct;36(10):1713–21.
Girard P, Beraud A, Sirvent A. Study of three complementary techniques for measuring cutaneous hydration in vivo in human subjects: NMR spectroscopy, transient thermal transfer and corneometry - application to xerotic skin and cosmetics: NMR-S, TTT and corneometry applied to xerotic skin and cosmetics. Skin Res Technol. 2000 Nov;6(4):205–13.
Spencer TS, Linamen CE, Akers WA, Jones HE. Temperature dependence of water content of stratum corneum. Br J Dermatol. 1975;93(2):159–64.
Egawa M, Hirao T, Takahashi M. In vivo Estimation of Stratum Corneum Thickness from Water Concentration Profiles Obtained with Raman Spectroscopy. Acta Derm Venereol. 2007;87(1):4–8.
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