Detection of the Timing of Human Skin Wounds by Immunohistochemical Analysis of CD14

Rasha I. Khedr, Fatma M. M. Badr El Dine, Heba M. K. El Dine Menesy, Amany A. Abdelatif, Azza A. Fouad

Abstract


Determination of time of injury is one of the most important topics in forensic autopsy. Several researches have been developed to estimate wound age, unfortunately with limited success. The aim of the present work was to evaluate the efficacy of Cluster of Differentiation 14 (CD14) as a reliable marker for estimating wound age. The study was conducted on forty bodies of victims with different types of wound and known infliction time. Skin samples were obtained during autopsy from the center of the wound. Sections from samples were histologically examined by H & E stain. Immunohistochemical staining was done using CD14 antibody and the staining density was evaluated semi-quantitatively. There was a statistically significant relation between wound age and percentage of CD14 expression. Expression of CD14 was 61.81±6.55 % in specimens from wounds aged less than 12 hours. It increased till reaching its maximum (96.40±3.78 %) for wounds aged between 1-3 days. Then it decreased dramatically to 14.80±3.49 % in wounds older than 3 days. CD14 is proved to be a reliable marker for estimating wound age. It gave best results in wounds aged between 1-3 days with an overall accuracy of 100%. Accordingly, it can be used to determine wound age in medicolegal practice.


Keywords


Forensic Science, Forensic Pathology, Wound Age, Immunohistochemistry, CD14

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References


Ishida Y, Kimura A, Nosaka M, Kuninaka Y, Shimada E, Yamamoto H. Detection of endothelial progenitor cells in human skin wounds and its application for wound age determination. Int J Legal Med.2015;129:1049-54. https://doi.org/10.1007/s00414-015-1181-7

Ishida Y, Kimura A, Takayasu T, Eisenmenger W, Kondo T. Detection of fibrocytes in human skin wounds

and its application for wound age determination. Int J Legal Med.2009; 123:299-304. https://doi.org/10.1007/s00414-009-0320-4

Kimura A, Ishida Y, Nosaka M, Shiraki M, Hama M, Kawaguchi T. Autophagy in skin wounds: a novel

marker for vital reactions. Int J Legal Med.2015;129:537-41.

https://doi.org/10.1007/s00414-015-1168-4

Grellner W, Madea B. Demands on scientific studies: vitality of wounds and wound age estimation. Forensic Sci Int.2007; 165:150-4. https://doi.org/10.1016/j.forsciint.2006.05.029

Kondo T. Timing of skin wounds. Leg Med.2007;9:109-14. https://doi.org/10.1016/j.legalmed.2006.11.009

Takamiya M, Fujita S, Saigusa K, Aoki Y. Simultaneous detection of eight cytokines in human dermal

wounds with a multiplex bead-based immunoassay for wound age estimation. Int J Legal Med.2008;122:143-8. https://doi.org/10.1007/s00414-007-0183-5

Van de Goot FR, Korkmaz HI, Fronczek J, Witte BI,Visser R, Ulrich MM. A new method to determine

wound age in early vital skin injuries: a probability scoring system using expression levels of Fibronectin,

CD62p and Factor VIII in wound hemorrhage. Forensic Sci Int. 2014; 244:128-35. https://doi.org/10.1016/j.forsciint.2014.08.015

Saukko P, Knight B. Knight’s Forensic Pathology.4th ed. New York: CRC Press Taylor & Francis Group;2016.

Fronczek J, Lulf R, Korkmaz HI, Witte BI, van de Goot FR, Begieneman MP. Analysis of inflammatory cells and mediators in skin wound biopsies to determine wound age in living subjects in forensic medicine. Forensic Sci Int. 2015; 247:7-13. https://doi.org/10.1016/j.forsciint.2014.11.014

Gauchotte G, Wissler MP, Casse JM, Pujo J, Minetti C,

Gisquet H. FVIIIra, CD15, and tryptase performance

in the diagnosis of skin stab wound vitality in forensic pathology. Int J Legal Med.2013; 127:957-65. https://doi.org/10.1007/s00414-013-0880-1

Wang LL, Zhao R, Liu CS, Liu M, Li SS, Li JY, et al.A fundamental study on the dynamics of multiple biomarkers in mouse excisional wounds for wound age estimation. J Forensic Leg Med .2016;39 :138- 46.

https://doi.org/10.1016/j.jflm.2016.01.027

Ishida Y, Kimura A, Takayasu T, Eisenmenger W ,Kondo T. Expression of oxygen-regulated protein 150

(ORP150) in skin wound healing and its application for wound age determination. Int J Legal Med.2008;

:409-14. https://doi.org/10.1007/s00414-008-0255-1

Kitchens RL. Role of CD14 in cellular recognition of bacterial lipopolysaccharides. Chem Immunol.

;74:61-82. https://doi.org/10.1159/00005875014. Tapping RI, Tobias PS. Soluble CD14-mediated

cellular responses to lipopolysaccharide.Chem Immunol.2000;74:108-21.

https://doi.org/10.1159/000058751

Hermansson C, Lundqvist A, Magnusson L, Ullström C, Bergström G, Mattsson L. Macrophage CD14 expression in human carotid plaques is associated with complicated lesions, correlates with thrombosis, and is reduced by angiotensin receptor blocker treatment. Int Immunopharmacol.2014;22: 318-23. https://doi.org/10.1016/j.intimp.2014.07.009

Zhou J, Ouyang X, Cui X, Schoeb TR, Smythies LE,Johnson MR, et al. Renal CD14 expression correlates with the progression of cystic kidney disease. Kidney Int. 2010;78(6):550-60. https://doi.org/10.1038/ki.2010.175

Friedrich K, Smit M, Brune M, GieseT, Rupp C,Wannhoff A. CD14 is associated with biliary stricture

formation. Hepatology.2016; 64(3):843-52. https://doi.org/10.1002/hep.28543

Hajian-Tilaki K. Receiver Operating Characteristic (ROC) Curve Analysis for Medical Diagnostic Test

Evaluation. Caspian J Intern Med.2013;4 (2): 627-35.19. Yagi Y, Murase T, Kagawa S, Tsuruya S, Nakahara A, YamamotoT. Immunohistochemical detection of CD14 and combined assessment with CD32B and CD68 for wound age estimation. Forensic Sci Int.2016;262:113-20. https://doi.org/10.1016/j.forsciint.2016.02.031

Frey EA, Miller DS, Jahr TG, Sundan A, Bazil V, Espevik T, et al. Soluble CD14 participates in the response of cells to lipopolysaccharide. J Exp Med.1992;176:1665-71. https://doi.org/10.1084/jem.176.6.1665

Krüger C, Schütt C, Obertacke U, Joka T, Müller FE, Knöller J, et al. Serum cd14 levels in polytraumatized and severely burned patients. Clin Exp Immunol.1991; 85 (2):297–301. https://doi.

org/10.1111/j.1365-2249.1991.tb05722.x

Kamada N, Hisamatsu T, Okamoto S, Chinen H, Kobayashi T, Sato T, et al. Unique CD14 intestinal macrophages contribute to the pathogenesis of Crohn disease via IL-23/IFN-gamma axis. J Clin Invest.2008; 118:2269-80. https://doi.org/10.1172/JCI34610

de Buhr MF, Hedrich HJ, Westendorf AM, Obermeier F, Hofmann C, Zschemisch NH, et al. Analysis of

Cd14 as a genetic modifier of experimental inflammatory bowel disease (IBD) in mice. Inflam Bowel

Dis.2009;15(12):1824-36. https://doi.org/10.1002/ibd.21030

Takeshita S, Nakatani K, Kawase H, Seki S, Yamamoto M, Sekine I, et al.The role of bacterial lipopolysaccharide-bound neutrophils in the pathogenesis of Kawasaki disease. J Infect Dis.1999;179(2):508-12. https://doi.org/10.1086/314600

Qubaja M, Marmey B, Le Tourneau A, Haiat S, Cazals-Hatem D, Fabiani B, et al. The detection of cd14 and cd16 in paraffin-embedded bone marrow biopsies is useful for the diagnosis of chronic myelomonocytic leukemia. Virchows Arch.2009; 454(4):411-9.

https://doi.org/10.1007/s00428-009-0726-x

Xu Y, McKenna RW, Karandikar NJ , Pildain AJ,Kroft SH . Flow cytometric analysis of monocytes

as a tool for distinguishing chronic myelomonocytic leukemia from reactive monocytosis. Am J Clin Pathol.2005;124:799-806. https://doi.org/10.1309/HRJ1-XKTD-77J1-UTFM

Sharma A, Khanna SK, Aggrawal A, Mandal AK. A histopathological study to determine the age of contusion. J Punjab Acad Forensic Med Toxicol.2010;10:17-9.

Vinay J , Harish S, Mangala GSR, Hugar BS. A study on postmortem wound dating by gross and histopathological examination of abrasions. Am J Forensic Med Pathol.2017;38(2):167-73. https://doi.org/10.1097/ AF.0000000000000314

Oehmichen M. Vitality and time course of wounds. Forensic Sci Int.2004;144:221-31.

https://doi.org/10.1016/j.forsciint.2004.04.057

Unmesh AK, Rema P. Histo-morphology of age of contusions: An autopsy study. NJMR.2012; 2(3): 339-42.

Kagawa S, Matsuo A, Yagi Y, Ikematsu K, Tsuda R,Nakasono I. The time-course analysis of gene expression during wound healing in mouse skin. Legal Med (Tokyo).2009; 11 (2):70-5. https://doi.org/10.1016/j.legalmed.2008.09.004




DOI: http://dx.doi.org/10.26735/16586794.2019.024

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