Determination of Psychoactive Mitragynine Drug in Suspected Kratom Species Collected from Various Geographical Areas in the Philippines: A Pilot Study on Existing Local Plant-based New Psychoactive Substance (NPS)

Atty. Severino P. Uy,, Shaila Jr, S. Seville, Jasmyne L. M. Jaranilla, Yvette K. S. Desamito, Reynalyn P. Barbacena, Ronald J. A. Narceda

Abstract


Kratom is a tropical tree indigenous to South East Asian countries and has been traditionally used by natives to increase work efficiency and treat selected illnesses. However, the United Nations Office on Drugs and Crime (UNODC) classified kratom, Mitragyna speciose, as a plant-based New Psychoactive Substance (NPS) that must be monitored worldwide, due to increasing reports of abuse. Many countries, including the Philippines, do not put restrictions on the said plant species including its major psychoactive drug, mitragynine. Under this prevailing provision, a research exploration was carried out to determine the distribution of kratom trees, locally known as “mambog”, in the Philippines and authenticate species identity of collected specimens through chemical determination of mitragynine and DNA analysis.

Various samples, specifically leaves, twigs, barks and roots, from claimed kratom species in selected regions of Luzon and the Mindanao Islands of the Philippines were sampled and preserved accordingly before subjecting them to instrumental analysis using Gas-Chromatograph Mass Spectrometer (GC-MS) and DNA barcoding.

During the field exploration, it was well documented that claimed kratom trees are mostly present in wetland areas at low altitudes, and sometimes co-exist with local bangkal (genus Nauclea) trees. Interestingly, while locals identified some of the collected species as kratom through botanical assessment, mitragynine was not detected in some selected sampling sites. Remarkably, among tree parts collected, only leaves and twigs showed evidence of mitragynine suggesting further disparity among kratom tree parts. On the other hand, DNA barcoding technique was utilized to discern the species level identities of claimed kratom trees in the field, which revealed that acquired plant specimens that were found positive for mitragynine were suggested to be Mitragyna speciosa, the specific kratom species being monitored by the UNODC.

Given that the present laboratory findings confirmed the existence of kratom Mitragyna speciosa in the country, it is of great importance for the Philippine government to implement strict monitoring and scientific evaluation on the potential threats of the said plant-based NPS. Preliminary results can also assist law makers to possibly develop regulations in the future, since this paper is a pilot forensic study for kratom found in the Philippines.


Keywords


Forensic Science, Plant-Based New Psychoactive Substances, Kratom, Mitragyna speciosa, Mitragynine

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References


Low JW, Leela A, Bhore SJ. The Kratom Plant [Mitragyna speciosa (Korth.)] Paradox: Beneficial or Detrimental? Res Highl 4Bs. 2016;4Bs: 84-89.

Casey CR, Conley T, Heise A, Thomas T, Ayres PR. Quantitative and Qualitative Analysis of Mitragynine

in Kratom (Mitragyna Speciosa) by GC-MS, LC-MS/ MS and UPLC-PDA. J Regul Sci. 2015;3(2):1-4.

Suwanlert S. A study of Kratom eaters in Thailand. Bulletin on narcotics. 1975;27(3):21-7.

Ranggasamy R, Ghafar ZA, Jean SW, Hussain NH, Japri N, Badron UH, Juan T, Wasiman MI, Ismail Z.

Herbal monograph methodology for identification of Mitragyna speciosa (Korth.) Havil. leaves. J Pharmacogn Phytochem. 2015;4(4):256.

Grewal KS. Observations on the pharmacology of mitragynine. J Pharmacol Exp Ther. 1932;46(3):251-71.

Jansen KL, Prast CJ. Ethnopharmacology of Kratom and the Mitragyna alkaloids. J Ethnopharmacol.

;23(1):115-9. https://doi.org/10.1016/0378-8741(88)90121-3

Bergen-Cico D, MacClurg K. Kratom (Mitragyna speciosa) use, addiction potential, and legal status. Neuropathology of Drug Addictions and Substance Misuse. 2016;89: 903-911. https://doi.org/10.1016/B978-0-12-800634-4.00089-5

United Nations Office on Drugs and Crime (UNODC), UNODC Early Warning Advisory on New Psychoactive Substances (NPS). Accessed: 31 December 2018, https://www.unodc.org/LSS/SubstanceGroup/Details/4b17fe10-91da-477c-bc3d-593136040668

Kronstrand R, Roman M, Thelander G, Eriksson A. Unintentional fatal intoxications with mitragynine and O-desmethyltramadol from the herbal blend Krypton. J Anal Toxicol. 2011;35(4):242-7.

https://doi.org/10.1093/anatox/35.4.242

Heather G, Rachel W, John D. Preliminary Analysis of FDA “Kratom-related” Deaths. Accessed: 31 December 2018,

https://theeye.eu/public/Psychedelics/Psychedelic%20Praxis%20Library%203.0/Collections%20by%20Substance/Kratom/2018%20-%20Preliminary%20Analysis%20of%20FDA%27s%20

%20Kratom-Related%20Deaths.pdf

Chittrakarn S, Penjamras P, Keawpradub N. Quantitative analysis of mitragynine, codeine, caffeine, chlorpheniramine and phenylephrine in a Kratom (Mitragynaspeciosa Korth.) cocktail using high-performance liquid chromatography. Forensic Sci Int. 2012;217(1-3):81-6. https://doi.org/10.1016/j.forsciint.2011.10.027

Philippines Kratom. Accessed: 31 December 2018,https://www.philippinesKratom.ph/

Alejandro GJ. The current status of the Philippine Rubiaceae. Philipp J Syst Biol. 2009 Apr 22;1(1):47-60.https://doi.org/10.3860/pjsb.v1i1.908

Jaipaew J, Padungchareon T, Sukrong S. PCR-reverse dot blot of the nucleotide signature sequences of matK for the identification of Mitragyna speciosa, a narcoticspecies. Plant Gene. 2018;14: 46-54. https://doi.org/10.1016/j.plgene.2018.04.005

Philippine Medicinal Plants, Mambog Mitragyna speciosa (Korth) Havil Kratom. Accessed: 31 December 2018, http://www.stuartxchange.org/Mambog.html

Brown PN, Lund JA, Murch SJ. A botanical, phytochemical and ethnomedicinal review of the genus Mitragyna korth: implications for products sold as Kratom. J Ethnopharmacol. 2017;202:302-25. https://doi.org/10.1016/j.jep.2017.03.020

Goh TB, Mordi MN, Khoo KH, Mansor SM. Comparison of the mitragynine content of aqueous extracts

from Mitragyna speciosa Korth leaves from two different geographical areas in Malaysia. As J Food Ag-Ind.

;6(3):142-8.

Mazerolle MJ. Peatlands and green frogs: A relationship regulated by acidity? Ecoscience. 2005;12 (1): 60-67. https://doi.org/10.2980/i1195-6860-12-1-60.1

Brown PN, Lund, JA, Murch, SJ. A botanical, phytochemical and ethnomedicinal review of the genus Mitragyna Korth: Implications for products sold as Kratom.

J Ethnopharmacol. 2017;202: 302-325. https://doi.org/10.1016/j.jep.2017.03.020

Almeria ML, Nuñeza OM. Amphibian diversity and endemism in the swamp forests of Agusan Marsh, Agusan Del Sur, Philippines. AES Bioflux. 2013;5 (1): 30- 48.

Chittrakarn S, Sawangjaroen K, Prasettho S, Janchawee B, Keawpradub N. Inhibitory effects of Kratom leaf extract (Mitragyna speciosa Korth.) on the rat gastrointestinal tract. Ethnopharmacol. 2008;116: 173-178. https://doi.org/10.1016/j.jep.2007.11.032.




DOI: http://dx.doi.org/10.26735/16586794.2019.025

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