Post-Mortem Analysis of Diazinon and its major Metabolite, 2-isopropyl-4- methyl-6-hydroxypyrimidine, in a Case of Fatal Diazinon Ingestion

Ibraheem M. Attafi, Magbool E oraiby, Mohsen M Fageeh, Ghassam A Shaikhain, Mohammed Y Albeishy, Ibrahim A Khardali, Mohammed A. Hakami

Abstract


This case report describes a detection and quantitation method
for diazinon and its major metabolite, 2-isopropyl-4-methyl-6-hydroxypyrimidine
(IMHP), in postmortem blood and tissue samples
from a fatal case of diazinon ingestion. Diazinon and IMHP were
extracted from postmortem samples with a liquid/liquid method
and analyzed by gas chromatography mass spectrometry (GC/MS).
By comparing to diazinon standard and matching the retention
time, diazinon was detected in two visceral organs, the stomach
(0.89 μg/g) and the small intestine (8.80 μg/g). The highest level
of diazinon was detected in the small intestine (8.80 μg/g), whereas
the highest amount of IMHP was noted in the kidney (0.84 Area %)
and bladder (0.75 Area %).
In conclusion, determination of IMHP in postmortem samples
could be used as an indicator for diazinon exposure, especially in
the case of delayed death; whereas, the small intestine could be
the best source of sample in diazinon assessment in cases of fatal
diazinon ingestion.


Keywords


Forensic Sciences, Diazinon, IMHP, Pesticides, GCMS, Postmortem, Forensic Toxicology.

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References


WHO. The World Health Organization recommended

classification of pesticides by hazard

and guidelines to classification 2009. 2010;

Available from: http://apps.who.int/iris/bitstre

am/10665/44271/1/9789241547963_eng.pdf.

Morgan MK, Stout DM, Jones PA, Barr DB. An observational

study of the potential for human exposures

to pet-borne diazinon residues following lawn applications.

Environ res. 2008;107(3):336-42. https://doi.

org/10.1016/j.envres.2008.03.004

Carlton EJ, Moats HL, Feinberg M, Shepard P, Garfinkel

R, Whyatt R, Evans D. Pesticide sales in lowincome,

minority neighborhoods. J Community

Health. 2004;29(3):231-44. https://doi.org/10.1023/

B:JOHE.0000022029.88626.f4

Kendall P. Public Health Considerations on Cosmetic

Use of Pesticides in BC 2013. Available from: http://

www.bccdc.ca/resource-gallery/Documents/Guidelines%

and%20Forms/Guidelines%20and%20Manuals/

Health-Environment/CosmeticPesticidesinBCwebfinal1.

pdf.

Hamad MH, Adeel AA, Alhaboob AA, Ashri AM, Salih

MA. Acute poisoning in a child following topical treatment

of head lice (pediculosis capitis) with an organophosphate

pesticide. Sudan J Paediatr. 2016;16(1):63.

Halle A, Sloas DD. Percutaneous organophosphate poisoning.

South Med J. 1987;80(9):1179-81. https://doi.

org/10.1097/00007611-198708090-00025

Poklis A, Kutz FW, Sperling JF, Morgan DP. A fatal diazinon

poisoning. Forensic Sci Int. 1980;15(2):135-40.

https://doi.org/10.1016/0379-0738(80)90152-8

Garfitt SJ, Jones K, Mason HJ, Cocker J. Exposure to

the organophosphate diazinon: data from a human volunteer

study with oral and dermal doses. Toxicol Lett.

;134(1-3):105-13. https://doi.org/10.1016/S0378-

(02)00178-9

Banerjee I, Tripathi S, Roy AS. Clinico-epidemiological

characteristics of patients presenting with organophosphorus

poisoning. N Am J Med Sci. 2012;4(3):147-50.

https://doi.org/10.4103/1947-2714.93884

Yurumez Y, Durukan P, Yavuz Y, Ikizceli I, Avsarogullari

L, Ozkan S, et al. Acute organophosphate

poisoning in university hospital emergency room patients.

Intern Med. 2007;46(13):965-9. https://doi.

org/10.2169/internalmedicine.46.6304

Harris LW, Fleisher JH, Innerebner TA, Cliff WJ,

Sim VM. The effects of atropine-oxime therapy on

cholinesterase activity and the survival of animals

poisoned with O,O-diethyl-O-(2-isopropyl-6-methyl-

-pyrimidinyl) phosphorothioate. Toxicol Appl Pharmacol.

;15:216-24. https://doi.org/10.1016/0041-

X(69)90150-1

DePalma AE, Kwalick DS, Zukerberg N. Pesticide

poisoning in children. JAMA. 1970;211(12):1979-81.

https://doi.org/10.1001/jama.1970.03170120025004

Morgan MK, Sheldon LS, Jones PA, Croghan CW, Chuang

JC, Wilson NK. The reliability of using urinary

biomarkers to estimate children’s exposures to chlorpyrifos

and diazinon. J Expo Sci Environ Epidemiol.

;21(3):280-90. https://doi.org/10.1038/jes.2010.11

Kiljanek T, Niewiadowska A, SEMENIUK S. Determination

of organophosphorus pesticide residues in the

liver by gas chromatography with flame photometric

detection. Bull Vet Inst Pulawy. 2011;55:731-6.

Dulaurent S, Gaulier JM, Blanc-Lapierre A,

Im-bert L, Lachatre G. Urinary determination of 2-isopropyl-

-methyl-6-hydroxypyrimidine in case of

non fatal poisoning with diazinon. Forensic Sci

Int. 2013;228(1-3):e20-4. https://doi.org/10.1016/j.

forsciint.2013.03.001

Klemmer HW, Reichert ER, Yauger WL, Jr. Five cases

of intentional ingestion of 25 percent diazinon with

treatment and recovery. Clin Toxicol. 1978;12(4):435-

https://doi.org/10.3109/15563657809150014

Mancini F, Jiggins JL, O’Malley M. Reducing the incidence

of acute pesticide poisoning by educating farmers

on integrated pest management in South India. Int J

Occup Environ Health. 2009;15(2):143-51. https://doi.

org/10.1179/oeh.2009.15.2.143

Tomokuni K, Hasegawa T, Hirai Y, Koga N. The tissue

distribution of diazinon and the inhibition of

blood cholinesterase activities in rats and mice receiving

a single intraperitoneal dose of diazinon. Toxicol.

;37(1-2):91-8. https://doi.org/10.1016/0300-

X(85)90115-5

Wu H, Evreux-Gros C, Descotes J. Diazinon toxicokinetics,

tissue distribution and anticholinesterase activity

in the rat. Biomed Environ Sci. 1996;9(4):359-69.

Lau C, Chung K, Kam C. Accidental organophosphate

insecticide poisoning: a fatal child case with

hazards to emergency department staff. HONG

KONG JEMERG ME. 2003;10(4):248-52. https://doi.

org/10.1177/102490790301000407

Iverson F, Grant D, Lacroix J. Diazinon metabolism in

the dog. Bull Environ Contam Toxicol. 1975;13(5):611-

https://doi.org/10.1007/BF01685188

Ellison CA, Tian Y, Knaak JB, Kostyniak PJ, Olson

JR. Human hepatic cytochrome P450-specific metabolism

of the organophosphorus pesticides methyl parathion

and diazinon. Drug Metab Dispos. 2011:dmd.

042572.




DOI: http://dx.doi.org/10.26735/16586794.2018.033

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