The questioned document examiners usually encounter cases related to handwritten documents for the determination of their source, origin, and authenticity. The legal documents usually involve handwriting or signatures executed using ballpoint pens. These components of the ballpoint pen inks can be analysed, both qualitatively and quantitatively, using several techniques. The present study aimed to analyse the red and black ballpoint pen inks using high performance thin layer chromatography and gas chromatography-mass spectrometry techniques. All samples have been completely differentiated using high performance thin layer chromatography. The discrimination potential of gas chromatography-mass spectrometry for red and black ballpoint pen inks was found to be 32.85% and 63.58% respectively. Classification of inks has been performed in two levels, that is, primary differentiation on the grounds of major components of the ink and subsequent differentiation on the basis of minor components. The validity of technique was tested in terms of repeatability and reproducibility. Reproducibility has been determined by repeating the procedure of repeatability on different days. The results have been evaluated in terms of relative standard deviation (RSD), which is <2% for both repeatability and reproducibility.

Barker K, Ramotowski R, Nwokoye J. The effect of

solvent grade on thin layer chromatographic analysis

of writing inks. Forensic Sci Int. 2016; 266: 139-47.

https://doi.org/10.1016/j.forsciint.2016.05.003

Sharma V, Kumar R. Fourier transform infrared spectroscopy

and high performance thin layer chromatography

for characterization and multivariate discrimination

of blue ballpoint pen ink for forensic applications. Vib

Spectro. 2017; 92:96-104. https://doi.org/10.1016/j.

vibspec.2017.05.006

Lee LC, Yunus ISM, Fuad WNSW, Ishak AA, Osman

K. Statistical Discrimination of Black Ballpoint

Pen Inks Using UltraPerformance Liquid Chromatography

with Principal Component Analysis. J Analyt

Chem.2015; 70 (3):374-7. https://doi.org/10.1134/

S1061934815030119

Sharma V, Kumar R. Dating of ballpoint pen writing

inks via spectroscopic and multiple linear regression

analysis: A novel approach. Microchemical J. 2017; 134:

-113. https://doi.org/10.1016/j.microc.2017.05.014

Chen H.S. Analysis of four ball point pen inks and two

dyes by thin layer chromatography with fluorescence

detection and matrix assisted laser desorption time of

flight and electrospray ionization mass spectrometry.

Forensic Sci J. 2005;4: 15-27.

da Silva Ferreira P, e Silva DF, Augusti R, Piccin E.

Forensic analysis of ballpoint pen inks using paper

spray mass spectrometry. Analyst. 2015;140(3):811-9.

https://doi.org/10.1039/C4AN01617C

Jones RW, McClelland JF. Analysis of writing inks on

paper using direct analysis in real time mass spectrometry.

Forensic Sci Int. 2013;231(1-3):73-81. https://doi.

org/10.1016/j.forsciint.2013.04.016

Weyermann C, Marquis R, Mazzella W, Spengler B.

Differentiation of blue ballpoint pen inks by laser desorption

ionization mass spectrometry and high‐performance

thin‐layer chromatography. J forensic sci.

;52(1):216-20. https://doi.org/10.1111/j.1556-

2006.00303.x

Senior S, Hamed E, Masoud M, Shehata E. Characterization

and dating of blue ballpoint pen inks using

principal component analysis of UV–Vis absorption

spectra, IR spectroscopy, and HPTLC. J forensic sci.

;57(4):1087-93. https://doi.org/10.1111/j.1556-

2012.02091.x

Calcerrada M, Garcia-Ruiz C. Analysis of questioned

document- A review. Analytical Chimica

Acta. 2015; 853: 143-166. https://doi.org/10.1016/j.

aca.2014.10.057

Maind SD, Chattopadhyay N, Gandhi C, Kumar SC,

Sudersanan M. Quantitative evaluation of europium in

blue ballpoint pen inks/offset printing inks tagged with

europium thenoyltrifluoroacetonate by spectrofluorometry

and ICP-AES. Sci Justice. 2008;48(2):61-6. https://

doi.org/10.1016/j.scijus.2007.09.007

Lee LC, Nunurung SM, Ishak AA. Feasibility of

HPTLC for the forensic analysis of red ballpoint pen

inks. The Malaysian J Analytical Sci. 2014; 97:14-19.

Lee LC, Nunurung SM, Ishak AA. Forensic analysis

of blue ballpoint pen inks on questioned documents by

HPTLC. The Malaysian J Analytical Sci. 2014; 18(2) :

-253.

Lee LC, Nunurung SM, Ishak AA. The analysis of dyes

in black ballpoint pen inks using HPTLC. The Malaysian

J Analytical Sci. 2014; 5(2): 22-26.

Hosu A, Pop B, Cimpoiu C. The forensic analysis of

pigments from some inks bye HTLC, Journal of Liquid

Chromatography and Related Technologies. 2015; 38:

-1112. https://doi.org/10.1080/10826076.2015.10

Aginsky VN. Using TLC and GC-MS to determine

whether inks came from the same manufacturing batch.

Journal of American Society of Questioned Document

Examiner. 1996; 9(1):19-27.

Laporte GM. The identification of 2-phenoxyethanol

in ballpoint inks using gas chromatography/mass spectrometry-

Relevance to ink dating. J forensic sci. 2004;

(1): 1-5. https://doi.org/10.1520/JFS2003217

Neumann C, Margot P. New perspective in the use of

ink evidence in forensic science- Part I. Development

of quality assurance process for forensic ink analysis by

HPTLC. Forensic Sci Int. 2009; 185: 29-37. https://doi.

org/10.1016/j.forsciint.2008.11.016

Senior S, Hamed E, Masoud M, Shehata E. Characterization

and dating of blue ballpoint pen inks using principal

component analysis of UV-Vis absorption spectra,

IR spectroscopy and HPTLC. J forensic sci. 2012;

(4): 1087-1093. https://doi.org/10.1111/j.1556-

2012.02091.x

Wayermann C, Marquis R, Mazzella W, Spengler B.

Differentiation of blue ballpoint pen inks by laser desorption

ionization mass spectrometry and high performance

thin layer chromatography. J forensic sci.

; 52(1): 216-220. https://doi.org/10.1111/j.1556-

2006.00303.x